The SARS-CoV-2 (COVID-19) IgM ELISA is intended for the qualitative determination of IgM class antibodies against SARS-CoV-2 in human serum or plasma (citrate, heparin) to support the diagnosis of COVID-19 disease and constitutes a supplement to direct pathogen detection.

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SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV- 2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.

The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labeled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.

More Information
SKU EC123M00
Test principle Enzyme-linked Immunosorbent Assay
Format Microtiter plate with 96 wells
Incubation Time 2 hours
Shelf Life (months) 6
Storage 2–8°C