Triclosan, Magnetic Particle ELISA, 100-test
Magnetic particle kit for for detection of Triclosan and Triclosan methyl.
"Magnetic particle kit for For detection of Triclosan and Triclosan methyl. Please refer to the specific procedures for water (groundwater, surface water, well water, effluent), and soil. Application procedures for other sample matrices can be obtained from Eurofins Abraxis. The Triclosan Assay applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of Triclosan and Triclosan methyl. The sample to be tested is added, along with paramagnetic particles attached with antibodies specific to Triclosan, to a disposable glass test tube and incubated for thirty (30) minutes . This is followed by the addition of a Triclosan enzyme conjugate. Both the Triclosan (which may be in the sample) and the enzyme labeled Triclosan derivative (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of a thirty minute (30) incubation period, a magnetic field is applied to hold the paramagnetic particles (with Triclosan and labeled Triclosan analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of Triclosan is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'- tetramethylbenzidine). The enzyme-labeled Triclosan analog bound to the Triclosan antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period of twenty (20) minutes, the reaction is stopped and stabilized by the addition of acid. Since the labeled Triclosan (conjugate) was in competition with the unlabeled Triclosan (sample) for the antibody sites, the color developed is inversely proportional to the concentration of Triclosan in the sample."