SENSISpec ELISA Mustard 48 wells

SENSISpec ELISA Mustard 48 wells

SENSISpec ELISA Mustard 96 wells

The SENSISpec Mustard ELISA represents a highly sensitive detection system for yellow mustard and is particularly capable of the quantification of residues in sausage, dressings, soups, cheese and mixed herbs. Due to high cross-reactivity the test is also suitable for the detection of brown mustard and black mustard.

Mustard belongs to the Brassica plants. With about 30-35% the fraction of proteins in mustard seed is very high. Some of these proteins are known for being allergenic, such as Sin a 1 and Bra j 1. These proteins are predominantly heat resistant making them stable to different production processes. In addition to brown mustard (Brassica juncea) and black mustard (Brassica nigra) primarily yellow mustard (Sinapsis alba) is used as an ingredient in many foods and food preparations.

For mustard allergic persons hidden mustard allergens in food are a critical problem. Already very low amounts of mustard can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, mustard allergic persons must strictly avoid the consumption of mustard or mustard containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. The sausage production process is a representative example. This explains why in many cases the existence of mustard residues in food cannot be excluded. For this reason sensitive detection systems for mustard residues in foodstuffs are required.

The SENSISpec Mustard ELISA represents a highly sensitive detection system for yellow mustard and is particularly capable of the quantification of residues in sausage, dressings, soups, cheese and mixed herbs. Due to high cross-reactivity the test is also suitable for the detection of brown mustard and black mustard.

More Information
SKU HU0030016
Test principle Sandwich enzyme immunoassay
Format Microtiter plate with 96 wells
Incubation Time 60 min (20/20/20 min)
Standards 0; 2, 6; 20; 60 ppm
Detection 450 nm (HRP + TMB)
Detection limit (LOD) 1 ppm
Quantitation Range 2 - 60 ppm
Cross-reactivities Brown mustard (seed) 26.5% Black mustard (seed) 32.5% Horseradish 0.0007% Garden cress 0.0009% Garden cress (seed) 1.5% Rape (seed) 15.5% Radish (seed) 31.2% Cabbage (seed) 29.2%
Shelf Life (months) 24
Storage 2–8°C