SENSISpec ELISA Mustard 96 wells
The SENSISpec Mustard ELISA represents a highly sensitive detection system for yellow mustard and is particularly capable of the quantification of residues in sausage, dressings, soups, cheese and mixed herbs. Due to high cross-reactivity, the test is also suitable for the detection of brown mustard and black mustard. The assay is validated on The BoltTM automated ELISA system.
Mustard belongs to the Brassica plants. With about 30-35% the fraction of proteins in mustard seed is very high. Some of these proteins are known for being allergenic, such as Sin a 1 and Bra j 1. These proteins are predominantly heat resistant making them stable to different production processes. In addition to brown mustard (Brassica juncea) and black mustard (Brassica nigra) primarily yellow mustard (Sinapsis alba) is used as an ingredient in many foods and food preparations.
For mustard allergic persons hidden mustard allergens in food are a critical problem. Already very low amounts of mustard can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, mustard allergic persons must strictly avoid the consumption of mustard or mustard containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. The sausage production process is a representative example. This explains why in many cases the existence of mustard residues in food cannot be excluded. For this reason sensitive detection systems for mustard residues in foodstuffs are required.
The SENSISpec Mustard ELISA represents a highly sensitive detection system for yellow mustard and is particularly capable of the quantification of residues in sausage, dressings, soups, cheese and mixed herbs. Due to high cross-reactivity the test is also suitable for the detection of brown mustard and black mustard.
|Test principle||Sandwich enzyme immunoassay|
|Format||Microtiter plate with 96 wells|
|Incubation Time||60 min (20/20/20 min)|
|Standards||0; 2, 6; 20; 60 ppm|
|Detection||450 nm (HRP + TMB)|
|Detection limit (LOD)||1 ppm|
|Quantitation Range||2 - 60 ppm|
|Cross-reactivities||Brown mustard (seed) 26.5% Black mustard (seed) 32.5% Horseradish 0.0007% Garden cress 0.0009% Garden cress (seed) 1.5% Rape (seed) 15.5% Radish (seed) 31.2% Cabbage (seed) 29.2%|
|Shelf Life (months)||24|