Pyraclostrobin, ELISA, 96 tests
The Pyraclostrobin ELISA is an immunoassay for the quantitative and sensitive screening of Pyraclostrobin. This test is suitable for the quantitative and/or qualitative screening of Pyraclostrobin in water or wine samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Pyraclostrobin by specific antibodies. Pyraclostrobin, when present in a sample and a Pyraclostrobin-HRP analog competes for the binding sites of the mouse anti-Pyraclostrobin antibodies in solution. The Pyraclostrobin antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Pyraclostrobin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.