Pyraclostrobin, ELISA, 96 tests
Immunoassay for the quantitative and sensitive screening of Pyraclostrobin.
The Pyraclostrobin ELISA is an immunoassay for the quantitative and sensitive screening of Pyraclostrobin. This test is suitable for the quantitative and/or qualitative screening of Pyraclostrobin in water or wine samples. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Pyraclostrobin by specific antibodies. Pyraclostrobin, when present in a sample and a Pyraclostrobin-HRP analog competes for the binding sites of the mouse anti-Pyraclostrobin antibodies in solution. The Pyraclostrobin antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Pyraclostrobin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.