Polybrominated Diphenyl Ether (PBDE), Magnetic Particle ELISA, 100-test
Magnetic particle kit for detection of Polybrominated Diphenyl Ether (PBDEs). Please refer to the attached specific procedures for water (groundwater, surface water, well water, effluent), and soil. Application procedures for other sample matrices can be obtained from Eurofins Abraxis. The PBDE Assay applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of PBDE. The sample to be tested is added, along with paramagnetic particles attached with antibodies specific to PBDE, to a disposable glass test tube. This is followed by the addition of an PBDE enzyme conjugate. Both the PBDE (which may be in the sample) and the enzyme labeled PBDE (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of a twenty minute (20) incubation period, a magnetic field is applied to hold the paramagnetic particles (with PBDE and labeled PBDE analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of PBDE is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'- tetramethylbenzidine). The enzyme-labeled PBDE analog bound to the PBDE antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period of twenty (20) minutes, the reaction is stopped and stabilized by the addition of acid. Since the labeled PBDE (conjugate) was in competition with the unlabeled PBDE (sample) for the antibody sites, the color developed is inversely proportional to the concentration of PBDE in the sample.