Penoxsulam, Magnetic Particle ELISA, 100 tests
Magnetic particle kit for detection and quantitation of Penoxsulam in water (groundwater, surface water, well water). For soil, crop, and food use contact the company for application bulletins and/or specific matrix validation guidelines. The Penoxsulam Kit applies the principles of enzyme-linked immunosorbent assay (ELISA) to the determination of Penoxsulam. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles attached with antibodies specific to Penoxsulam. At this point, a competitive reaction occurs between the Penoxsulam which may be in the sample and the enzyme-labeled Penoxsulam analog for the antibody binding sites on the magnetic particles. The reaction is allowed to continue for forty-five (45) minutes. At the end of the incubation period, a magnetic field is applied to hold in the test tube the paramagnetic particles (with Penoxsulam and labeled Penoxsulam bound to the antibodies on the particles, in proportion to their original concentration), and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of Penoxsulam is detected by adding the Color Solution, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'-tetramethylbenzidine). The enzyme-labeled Penoxsulam bound to the Penoxsulam antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of a diluted acid (Stopping Solution). Since the labeled Penoxsulam (conjugate) was in competition with the unlabeled Penoxsulam (sample) for the antibody sites, the color developed is inversely proportional to the concentration of Penoxsulam in the sample.