PCBs Higher Chlorinated, Magnetic Particle ELISA, 100-test
Magnetic particle kit for detection of Polychlorinated Biphenyls (PCBs). Please refer to the attached specific procedures for water (groundwater, surface water, well water, effluent), and soil. Application procedures for other sample matrices can be obtained from Eurofins Abraxis. The Abraxis PCB Assay applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of PCB. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles with antibodies specific to PCB attached. Both the PCB (which may be in the sample) and the enzyme labeled PCB (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of an incubation period, a magnetic field is applied to hold the paramagnetic particles (with PCB and labeled PCB analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of PCB is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5'-tetramethylbenzidine). The enzyme-labeled PCB analog bound to the PCB antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of acid. Since the labeled PCB (conjugate) was in competition with the unlabeled PCB (sample) for the antibody sites, the color developed is inversely proportional to the concentration of PCB in the sample.