PCBs Higher Chlorinated, ELISA, 96-test
The PCB ELISA is an immunoassay for the quantitative and sensitive screening of Polychlorinated Biphenyls (PCBs). This test is suitable for the quantitative and/or qualitative screening of PCBs in water and soil samples (please refer to the appropriate technical bulletins for additional matrices procedures). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Polychlorinated Biphenyls by specific antibodies. The PCBs, when present in a sample, and a PCB-HRP analogue compete for the binding sites of anti-PCB antibodies in solution. The PCB antibodies are then bound by a second antibody immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of PCBs present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.