Vip3A ELISA Kit

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eCry3.1Ab ELISA kit

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PAT/Pat ELISA Kit

PAT/pat ELISA test kit is intended to be used for the qualitative detection of Liberty Link PAT/pat protein in individual leaf/seed samples. The kit detects phosphinothricin acetyltransferase enzyme (PAT) coded for by the pat gene in corn & soybean leaf or seed. The total incubation time of the assay is 60 minutes. Liberty link PAT from the Bar gene found in Starlink Corn, LL25 cotton and In Vigor Canola is not detected by this kit.

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Different quantities are also available in 10/50/100 plate formats. 
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An antibody specific to the PAT/pat protein molecule is immobilized on the microwell plates and second antibody specific for PAT/pat molecule is conjugated with horse radish peroxidase (HRP). When the sample extracts are added to the microtiter wells, the PAT/pat protein from the sample binds to the antibody immobilized in the well. This binding is subsequently detected by the addition of the enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the amount of PAT/pat protein present in the sample. The reaction is terminated by addition of stop solution. Absorbance is measured on a plate reader.
More Information
SKU AID 027
No. of tests 96
Specificity Qualitative detection of Liberty Link PAT/pat protein in individual leaf/seed samples
Relevant species Cotton/Corn leaf and seed
Cross-reactivities The PAT/pat ELISA kit does not recognize Cry1F, Cry2A, Cry9C, Cry1Ac/Ab or CP4EPSPS
Assay time 60 min
Storage 2-8 °C