Cry1Ac/Cry2A combined ELISA kit

Cry1Ac/Cry2A combined ELISA kit

Vip3A ELISA Kit

Vip3A ELISA Kit

PAT/Bar ELISA Kit

PAT/Bar ELISA test kit is intended to be used for the qualitative detection of Liberty Link PAT/Bar protein in individual leaf/seed samples. The kit detects phosphinothricin acetyltransferase enzyme (PAT) coded for by the Bar gene in leaf or seed. The kit can be used for canola, rice, cotton and mustard leaf or seed sample.

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Different quantities are also available in 10/50/100 plate formats. 
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An antibody specific to the PAT/Bar protein molecule is immobilized on the microwell plates and second antibody specific for PAT/Bar molecule is conjugated with horse radish peroxidase (HRP). When the sample extracts are added to the microtiter wells, the PAT/Bar protein from the sample binds to the antibody immobilized in the well. This binding is subsequently detected by the addition of an enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the amount of PAT/Bar protein present in the sample. The reaction is terminated by the addition of stop solution. Absorbance is measured on a plate reader.
More Information
SKU AID 018
No. of tests 96
Specificity Qualitative detection of Liberty Link PAT/Bar protein in individual leaf/seed samples
Relevant species Cotton/Corn/Canola/Mustard individual leaf/seed samples
Cross-reactivities The PAT/Bar ELISA kit does not recognize Cry1F, Cry2A, Cry9C, Cry1Ac/Ab or CP4EPSPS
Assay time 75 min
Storage 2-8 °C