Organophosphate/Carbamate (OP/C), ELISA, 96 tests
Immunoassay for qualitative screening of a wide range of organophosphate (including thiophosphate) and carbamate pesticides.
The Abraxis Organophosphate/Carbamate (OP/C) plate assay is for the immunoassay for qualitative screening of a wide range of organophosphate (including thiophosphate) and carbamate pesticides. This test is suitable for the qualitative screening of water samples (drinking water, groundwater, surface water, and well water). The test can also be used for the testing of collected dislodgeable residues from surface wash as well as pesticide residues prepared from dried extracts (please contact Eurofins Abraxis technical services for application bulletins and/or specific matrix validation guidelines). Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The Organophosphate/Carbamate plate assay is a qualitative colorimetric assay for the detection of organophosphates and carbamates, based on a modification of their inhibition of the enzyme Acetylcholinesterase (Ach-E). Ach-E hydrolyzes acetylthiocholine (ATC), which reacts with 5,5’-Dithio-bis(2-Nitrobenzoic Acid) [DTNB] to produce a yellow color which is then read at 405 nm. If organophosphate or carbamate pesticides are present in a sample, they will inhibit Ach-E, reducing or eliminating color formation, depending on concentration. Detection limits of the various OP/C pesticides differ depending on their ability to inhibit the enzyme. If it has been established that only a single OP/C is present, the test can be used in conjunction with appropriate standards for quantitative testing.