Nitroimidazoles ELISA kit v2, ELISA, 96 det.

ELISA test kit for the detection of nitroimidazoles.
ELISA test kit for the detection of nitroimidazoles.
More Information
SKU AB005
Assay principle Microplate competitive enzyme immunoassay.
Format Microtiter plate with 96 wells
Assay time 120 min
Analysable samples Milk, Honey
Detection limit (LOD)
  • Milk - Extraction
  • Honey - Neutralization, Centrifugation, Evaporation, Resuspension, Extraction, Centrifugation
Sample preparation
  • Muscle, Egg, Milk (Method I, High Sensitivity) - Homogenization, (Skimming for milk), Solvent Extractin, Centrifugation, Evaporation
  • Muscle, Egg, Honey (Method II, Solvent Free) - Homogenization, Buffer Extraction (Acid Hydrolysis for Honey), Centrifugation, Filtration
  • Milk (Method II) - Centrifugation, Dilution
  • Honey (Method III) - Acidic Hydrolysis, Purification on SPE Column, Elution, Evaporation
  • Feed - Grinding, Solvent Extraction, Dilution
Specificity
  • Dimetridazole 216%
  • Metronidazole 100%
  • Ronidazole 82%
  • Secnidazole 20%
  • Ornidazole 18%
  • Tinidazole <6%

Test Instructions