Solvent-free analysis: new ELISA DON kits from Eurofins Tecna
Eurofins Tecna is pleased to announce the new ELISA H2DON and B ZERO H2DON kits. These kits don’t require the use of any solvent in the preparation of the sample.
The kits are two fast (20 minutes), quantitative, sensitive immunoenzymatic assays in microplates (200 ppb detection limit), with a wide measuring range (up to 8 ppm), accurate and precise.
B ZERO H2DON kit uses the same reagents as the H2DON kit, but the calibrators are not included in the kit and should not be loaded. The dosage curve is virtually supplied in the kit certificate and can be used for the entire shelf-life of the product for the calculation of the sample dose. The wells that are normally dedicated for calibrators are saved with using this kit.
The kit has already been validated on whole grain maize, wheat and durum wheat. A performance data sheet is available with the results obtained by our researchers regarding the definition of specificity, sensitivity, accuracy and precision.
Tecna has also participated in the first proficiency test on maize matrix organized by the company Test Veritas. The sample, naturally contaminated for DON, had an assigned value of 851.29 ppb of vomitoxin. The value found by Tecna and provided to the organization is 920 ppb, which corresponds to a recovery of 108% and a z-score of 0.40, an excellent result.