Microcystins/Nodularins (ADDA) Serum, ELISA 96 tests
Immunoassay for the quantitative and sensitive detection of Microcystins and Nodularins in human serum samples.
For Research Use Only; Not for Use in Diagnostic Procedures.
The Microcystins ELISA for Serum is an immunoassay for the quantitative and sensitive detection of Microcystins and Nodularins in human serum samples. For additional serum types (canine, etc.), please see the appropriate technical bulletin. Sample preparation and dilution is required prior to analysis. Positive samples should be confirmed by HPLC, protein phosphatase assay, or other conventional methods. The test is a direct competitive ELISA which detects the presence of Microcystins and Nodularins in human serum. It is based on the recognition of Microcystins, Nodularins, and their congeners by a monoclonal antibody. Microcystins, Nodularins, and their congeners, when present in a sample, and a Microcystins-HRP analog competes for the binding sites of anti-Microcystins antibodies in solution. The Microcystins antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the plate. After a washing step and the addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of the Microcystins/Nodularins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.