Microcystins/Nodularins (ADDA) SAES, ELISA, 96 tests
The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples.
The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples. The test is an indirect competitive ELISA for the congener-independent detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific biotinylated antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the biotinylated anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a streptavidin-HRP is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
|No. of tests||96|
|Detection limit||0.016 ppb (μg/L)|
|Kit components||1. Microtiter plate (12 X 8 strips) coated with an analog of Microcystins conjugated to a protein 2. Standards: 0, 0.05, 0.15, 0.4, 1.5, 5.0 ppb; 1 mL each 3. Control: 0.75 ± 0.185 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Microcystins-ADDA SAES Antibody Solution, 6 mL 6. Microcystins-ADDA SAES Conjugate Solution, 12 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 6 mL|