Microcystins/Nodularins (ADDA) (EPA ETV) (EPA Method 546), ELISA, 96 tests
The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples.
The Microcystins/Nodularins (ADDA) ELISA is an immunoassay for the quantitative and sensitive congener-independent detection of Microcystins and Nodularins in water samples. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a second antibody-HRP label is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
|No. of tests||96|
|Max no. of samples||41|
|Detection limit||0.10 ppb (μg/L)|
|Time to Result||3h|
|Kit components||1. Microtiter plate (12 X 8 strips) coated with an analog of Microcystins conjugated to a protein 2. Standards: 0, 0.15, 0.40, 1.0, 2.0, 5.0 ppb; 1 mL each 3. Control: 0.75 ± 0.185 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Antibody Solution, 6 mL 6. Anti-Sheep-HRP Conjugate Solution, 12 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 6 mL|