I'screen SULFA QL, ELISA, 96 det.

ELISA test kit for the detection of sulphonamides.

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SKU	HU0050015
Assay principle	Microplate competitive enzyme immunoassay.
Format	Microtiter plate with 96 wells
Assay time	75 min
Analysable samples	Milk, Muscle, Honey, Egg
Detection limit (LOD)	Muscle, Milk, Egg, Honey (Muscle high sensitivity procedure) Sulfamerazine - <1 ppb (<1 ppb) Sulfamonomethoxine - <1 ppb (<1 ppb) Sulfadiazine - <1 ppb (<1 ppb) Sulfachloropyridazine - <1 ppb (<1 ppb) Sulfamethoxypyridazine - <1 ppb (<1 ppb) Sulfamethoxydiazine - 1 ppb (<1 ppb) Sulfadimethoxine - 1 ppb (<1 ppb) Sulfaquinoxaline - 1 ppb (<1 ppb) Sulfathiazole - 5 ppb (<1 ppb) Sulfamethizole - 5 ppb (<1 ppb) Sulfamethazine - 10 ppb (1 ppb) Sulfamethoxazole - 10 ppb (1 ppb) Sulfisoxazole - 20 ppb (2 ppb) Sulfapyridine - 20 ppb (2 ppb) Sulfadoxine - 50 ppb (5 ppb)
Sample preparation	Muscle, Egg - Homogenization, Buffer Extraction, Centrifugation, Filtration Muscle (High Sensitivity Procedure) - Homogenization, Solvent Extraction, Centrifugation, Evaporation Milk - Centrifugation, Dilution Honey - Acidic Hydrolysis, Dilution
Specificity	Sulfamerazine >100% Sulfamonomethoxine >100% Sulfadiazine >100% Sulfachloropyridazine >100% Sulfamethoxypyridazine >100% Sulfamethoxydiazine >100% Sulfadimethoxine >100% Sulfaquinoxaline >100% Sulfathiazole 100% Sulfamethizole 70% Sulfamethazine 25% Sulfamethoxazole 22% Sulfisoxazole 12% Sulfapyridine 9% Sulfadoxine 6% Sulfacetamide 2.5% Sulfaphenazole <2% Sulfabenzamide <2% Sulfaguanidine <1% Sulfanilamide <1%

Test Instructions