GMOScreen RT 35S/NOS/ABII IPC (NR)

GMOScreen RT 35S/NOS/ABII IPC (NR)

GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC

The GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC contains all reagents for 3 x 96 RT-PCR separate reactions for detection of three regulatory DNA sequences often used in GMO production with internal positive control (IPC):

1) the CaMV 35s promoter of the Cauliflower Mosaic Virus 
2) the FMV 34s promoter of the Figwort Mosaic Virus
3) the nos terminator from the nopalin synthetase gene of Agrobacterium tumefaciens

The GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC contains all reagents for 3 x 96 RT-PCR separate reactions for detection of three regulatory DNA sequences often used in GMO production with internal positive control (IPC):

1) the CaMV 35s promoter of the Cauliflower Mosaic Virus 
2) the FMV 34s promoter of the Figwort Mosaic Virus
3) the nos terminator from the nopalin synthetase gene of Agrobacterium tumefaciens

More Information
SKU 5421220303
No. of tests 3 x 96
Max no. of samples 3 x 45
Specificity Cauliflower Mosaic Virus, the NOS terminator from the nopalin synthetase, the 34S promoter from the Figwort Mosaic Virus
Relevant species All GM plant species
Detection limit (LOD) No
Time to Result ~2.5 h (~90 min PCR duration)
PCR cycler platforms ABI 7000, 7300, 7500, 7700, 7900, MX3000P or MX3005P, Bio-Rad iCycler iQ5 or equivalent real-time PCR cycler
Kit components

1) Qualitative real-time MasterMixes for 35S and NOS and FMV, composed of BasicMix and OligoMix each. Mix prior to use in empty vials (labels included)

2) Positive control DNA

3) 150 µL DNA stabilization buffer (for NTCs)

Store all reagents light protected at -20°C