GMOScreen RT IPC (NR) ocs-terminator / Laurical™ / oxy-235 Canola

GMOScreen RT IPC (NR) ocs-terminator / Laurical™ / oxy-235 Canola

GMOScreen RT IPC (NR) EPSPS/NOS/pat(syn)

GMOScreen RT IPC (NR) EPSPS/NOS/pat(syn)

GMOScreen RT IPC (LR) EPSPS/NOS/pat(syn)

The real-time detection kit GMOScreen RT IPC (LR/NR) EPSPS/NOS/pat(syn) provides materials for the qualitative detection of the following targets:

  • - the EPSPS modification
  • - the nos terminator from Agrobacterium tumefaciens
  • - the modified pat gene from Streptomyces viridochromogenes

The kit detects the target sequence in food, feed and seed sample types from which DNA in sufficient quality and quantity can be extracted. Detection of this sequence strongly indicates the presence of GM material.

An internal positive control (IPC) contained in the master mix is amplified in parallel and indicates inhibition, if it occurs, thus avoiding false-negative/inhibited results for every individual sample. The kit is part of Eurofins GeneScan Technologies´ comprehensive product line for screening, identification and quantification of genetically modified organisms, called GMOScreen, GMOIdent and GMOQuant. Please note, that the naturally occurring Agrobacterium tumefaciens, if contained in the sample, may produce a positive result in the nos terminator system. In case of questionable positive results (for example if no GMO can be identified following a positive screening result), further tests are recommended.
More Information
SKU 5421228701
No. of tests 96
Max no. of samples 46
Specificity NOS TERMINATOR FROM AGROBACTERIUM TUMEFACIENS AND THE MODIFIED PAT-GENE FROM STREPTOMYCES VIRIDOCHROMOGENES
Relevant species Canola/Rapeseed
Detection limit (LOD) The absolute limit of detection for the method has been validated as LODabs = 5 DNA target copies of EPSPS/NOS/pat(syn) per reaction.
PCR cycler platforms The GMOScreen RT IPC (LR/NR) EPSPS/NOS/pat(syn) kit is considered as fit for purpose for the following real-time PCR cyclers: - Bio-Rad CFX96 Touch™ - Agilent AriaMx™ - Agilent Mx3005P™, Mx3000P™ - Applied Biosystems® ABI 7500, ABI 7500 Fast (in standard mode)
Kit components The GMOScreen RT IPC (LR/NR) EPSPS/NOS/pat(syn) kits contain the following reagents for 96 reactions (excess volume included): - 2x Qualitative real-time master mixes for EPSPS/NOS/pat(syn) detection composed of: - 650 μL Basic Mix BM EFGi TP 2x (NR) 2.5U (NR) GS-P-26.014 - 390 μL Oligo Mix QL RT (LR/NR) IPC • GS-P-09.182 • EPSPS/NOS/pat(syn) Mix prior to use in empty vial as indicated below (labels included)! - 2x positive control DNA: 50 μL plasmid DNA pGS-P-09.182 (10 copies/μL) - 1x DNA stabilisation buffer (for NTCs), 150 μL