GMOScreen RT IPC CsVMV / CV127 / DP305423-1 Soy (NR) for CFX96 and MX3005P/MX3000P
The GMOScreen RT IPC CsVMV/CV127/DP305423-1 Soy real-time detection kits provide materials for the qualitative detection of the following targets:
- CsVMV/pat(syn) modification
- Event CV127 recombination site
- Event DP-305423-1
The kit detects the target sequence in food, feed and seed sample types from which DNA in sufficient quality and quantity can be extracted. Detection of this sequence strongly indicates the presence of GM material.
The method utilized in this kit uses the GMO PCR system described in the method published and validated by the EURL of the European Commission.
- Event DP-305423-1
An internal positive control (IPC) contained in the master mix is amplified in parallel and indicates inhibition, if it occurs, thus avoiding false-negative/inhibited results for every individual sample.
The kit is part of Eurofins GeneScan Technologies´comprehensive product line for screening, identification and quantification of genetically modified organisms, called GMOScreen, GMOIdent and GMOQuant.
|No. of tests||96|
|Max no. of samples||46|
|Specificity||CSVMV/PAT(SYN) MODIFICATION, THE EVENT CV127 RECOMBINATION SITE AND EVENT DP-305423-1 WITH INTERNAL POSITIVE CONTROL (IPC)|
|Detection limit (LOD)||To achieve a limit of detection of 0.01% target copies analyse 23 ng/μL of soybean DNA (115 ng/reaction).|
|PCR cycler platforms|| Bio-Rad CFX96 Touch™ Agilent AriaMx™ Agilent Mx3005P™, Mx3000P™ Applied Biosystems® ABI 7500, ABI 7500 Fast (in standard mode)|
|Kit components||The GMOScreen RT IPC CsVMV/CV127/DP305423-1 Soy kits contain the following reagents for 96 reactions (excess volume included): - 2x Qualitative real-time master mixes for CsVMV/CV127/DP305423-1 detection composed of: - 650 μL Basic Mix BM EFGi TP 2x (NR) 1.5U (NR) GS-P-26.012 - 390 μL Oligo Mix QL RT (LR) IPC • GS-P-09.139 • CsVMV/CV127/ DP305423-1 Mix prior to use in empty vial as indicated below (labels included)! - 2x positive control DNA: 50 μL plasmid DNA pGS-P-09.139 (10 copies/μL) - 1x DNA stabilisation buffer (for NTCs), 150 μL|