GMOScreen RT IPC CsVMV / CV127 / DP305423-1 Soy (LR) for ABI 7500 (Fast)
The GMOScreen RT IPC CsVMV/CV127/DP305423-1 Soy real-time detection kits provide materials for the qualitative detection of the following targets:
- CsVMV/pat(syn) modification
- Event CV127 recombination site
- Event DP-305423-1 The method utilized in this kit uses the GMO PCR system described in the method published and validated by the EURL of the European Commission.
- Event DP-305423-1
An internal positive control (IPC) contained in the master mix is amplified in parallel and indicates inhibition, if it occurs, thus avoiding false-negative/inhibited results for every individual sample.
The kit is part of Eurofins GeneScan Technologies´ comprehensive product line for screening, identification and quantification of genetically modified organisms, called GMOScreen, GMOIdent and GMOQuant.
| SKU | 5421227203 |
|---|---|
| No. of tests | 96 |
| Max no. of samples | 46 |
| Specificity | CSVMV/PAT(SYN) MODIFICATION, THE EVENT CV127 RECOMBINATION SITE AND EVENT DP-305423-1 WITH INTERNAL POSITIVE CONTROL (IPC) |
| Relevant species | Soy |
| Detection limit (LOD) | To achieve a limit of detection of 0.01% target copies analyse 23 ng/μL of soybean DNA (115 ng/reaction). |
| PCR cycler platforms | The GMOScreen RT IPC CsVMV/CV127/DP305423-1 Soy kits are considered as fit for purpose for the following real-time PCR cyclers: - Bio-Rad CFX96 Touch™ - Agilent AriaMx™ - Agilent Mx3005P™, Mx3000P™ - Applied Biosystems® ABI 7500, ABI 7500 Fast (in standard mode) |
| Kit components | The GMOScreen RT IPC CsVMV/CV127/DP305423-1 Soy kits contain the following reagents for 96 reactions (excess volume included): - 2x Qualitative real-time master mixes for CsVMV/CV127/DP305423-1 detection composed of: - 650 μL Basic Mix BM EFGi TP 2x (NR) 1.5U (NR) GS-P-26.012 - 390 μL Oligo Mix QL RT (LR) IPC • GS-P-09.139 • CsVMV/CV127/ DP305423-1 Mix prior to use in empty vial as indicated below (labels included)! - 2x positive control DNA: 50 μL plasmid DNA pGS-P-09.139 (10 copies/μL) - 1x DNA stabilisation buffer (for NTCs), 150 μL |
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IFU_GMOScreen RT-IPC-CsVMV-CV127-DP305423-1 Soy_5421227104_V4.2.pdf Size: (686.15 KB)