GMOScreen RT 35S/NOS/ABII IPC (LR)

GMOScreen RT 35S/NOS/ABII IPC (LR)

GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC

GMOScreen RT (LR/HR+) 35S/IPC and NOS/IPC and FMV/IPC

GMOScreen RT 35S/NOS/ABII IPC (NR)

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

The screening combination 35S/NOS/FMV covers a broad spectrum of genetically modified organisms but several important GMOs are still missing. The AgroBorder II assay offers much better coverage of relevant GMOs than the FMV-promoter test.

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

Benefits

  • All variants of the CaMV 35S promoter are detected
  • The AgroBorder II screening covers more commercialized GMOs than the FMV promoter detection
  • Multiplex kit for 45 samples per 96 well plate, three results per sample
  • No false-negative results: inhibition control (internal positive control; IPC) included in every reaction
  • Can be used flexibly with common DNA extraction methods
  • More Information
    SKU 5421227102
    No. of tests 96
    Max no. of samples 45
    Specificity Cauliflower Mosaic Virus (CaMV) 35S promoter, A. tumefaciens nos terminator, AgroBorder II-Element
    Relevant species All GM plant species
    Detection limit (LOD) LODrel ≤ 0.01%, LODabs ≤ 10 copies
    Time to Result ~2.5 h (~90 min PCR duration)
    PCR cycler platforms Agilent Mx3005P/Mx3000P, Agilent AriaMxTM, Bio-Rad CFX96 TouchTM and LightCycler® 480
    Kit components 2x (20x) Qualitative real-time MasterMix GS-P-09.121 (IPC) for 35S/NOS/ABII detection, each composed of
    • 650 µL BasicMix QL RT IPC (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U
    • 390 µL OligoMix QL RT IPC (LR or NR) GS-P-09.121 35S/NOS/ABII
    2x (20) 50 µL positive control DNA: plasmid DNAs pGE-P-09.121 (35S+NOS+ABII) (10 copies/µL) 1x (10x) 150 µL DNA stabilization buffer (for NTCs)