GMOScreen RT IPC (NR) 35S/NOS/FMV

GMOScreen RT IPC (NR) 35S/NOS/FMV

GMOScreen RT 35S/NOS/ABII IPC (NR)

GMOScreen RT 35S/NOS/ABII IPC (NR)

GMOScreen RT 35S/NOS/ABII IPC (LR)

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

The screening combination 35S/NOS/FMV covers a broad spectrum of genetically modified organisms but several important GMOs are still missing. The AgroBorder II assay offers much better coverage of relevant GMOs than the FMV-promoter test.

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

Benefits

  • All variants of the CaMV 35S promoter are detected
  • The AgroBorder II screening covers more commercialized GMOs than the FMV promoter detection
  • Multiplex kit for 45 samples per 96 well plate, three results per sample
  • No false-negative results: inhibition control (internal positive control; IPC) included in every reaction
  • Can be used flexibly with common DNA extraction methods
More Information
SKU 5421227101
No. of tests 96
Max no. of samples 45
Specificity Cauliflower Mosaic Virus (CaMV) 35S promoter, A. tumefaciens nos terminator, AgroBorder II-Element
Relevant species All GM plant species
Detection limit (LOD) LODrel ≤ 0.01%, LODabs ≤ 10 copies
Time to Result ~2.5 h (~90 min PCR duration)
PCR cycler platforms Applied Biosystems® 7500/Fast
Kit components 2x (20x) Qualitative real-time MasterMix GS-P-09.121 (IPC) for 35S/NOS/ABII detection, each composed of
  • 650 µL BasicMix QL RT IPC (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U
  • 390 µL OligoMix QL RT IPC (LR or NR) GS-P-09.121 35S/NOS/ABII
2x (20) 50 µL positive control DNA: plasmid DNAs pGE-P-09.121 (35S+NOS+ABII) (10 copies/µL) 1x (10x) 150 µL DNA stabilization buffer (for NTCs)