Glyphosate, ELISA, 96 tests
The Glyphosate ELISA is an immunoassay for the quantitative and sensitive screening of Glyphosate in water, beverage, food, and feed samples. The test is a direct competitive ELISA based on the recognition of Glyphosate by polyclonal antibodies. The sample to be tested is derivatized and then added to microtiter wells coated with goat anti-rabbit antibodies. A rabbit anti-Glyphosate antibody solution is added to the wells with the derivatized samples and allowed to incubate for 30 minutes. The Glyphosate enzyme conjugate is then added and a competitive reaction occurs between the Glyphosate, which may be present in the sample, and the enzyme-labeled Glyphosate for the binding sites of the rabbit anti-Glyphosate antibodies bound by the goat anti-rabbit antibodies immobilized on the microtiter plate. The reaction is allowed to continue for 60 minutes. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Glyphosate present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
|No. of tests||96|
|Detection limit||0.05 ppb (μg/L)|
|Kit components||1. Microtiter plate coated with a secondary antibody (anti-rabbit) 2. Glyphosate Antibody Solution, 6 mL 3. Glyphosate Conjugate Solution, 6 mL 4. Glyphosate Standards: 0, 0.075, 0.20, 0.5, 1.0, 4.0 ppb; 2 mL each 5. Control at 0.75 ± 0.2 ppb, 2 mL 6 Diluent/Zero Standard (Sample Diluent), 30 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 16 mL 9. Stop Solution, 12 mL 10. Assay Buffer, 125 mL 11. Derivatization Reagent, 3 vials, 100 μL each 12. Derivatization Reagent Diluent, 3 vials, 4 mL each|