Fluoroquinolones, ELISA, 96 tests
The Fluoroquinolones ELISA is an immunoassay for the detection of Fluoroquinolones. This test is suitable for the quantitative and/or qualitative detection of Fluoroquinolones in contaminated samples including water, fish, shrimp and honey. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Fluoroquinolones by specific antibodies. Fluoroquinolones, when present in a sample, and a Fluoroquinolone-enzyme conjugate compete for the binding sites of rabbit anti-Fluoroquinolone antibodies in solution. The Fluoroquinolone antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Fluoroquinolones present in the sample. The color reaction is stopped after a specified time and the color is evaluated using a microplate ELISA photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.