Domoic Acid (ASP), ELISA, 96-test
The Domoic Acid (ASP) ELISA is an immunoassay for the quantitative and sensitive detection of Domoic Acid in water and seawater samples as well as shellfish samples. Domoic Acid is one of the toxins associated with amnesiac shellfish poisoning (ASP). For shellfish and marine water samples, a sample preparation is required as described in the Users Guide. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Domoic Acid by specific antibodies. Domoic Acid, when present in a sample, and a Domoic Acid-protein analogue immobilized on the microtiter plate compete for the binding sites of the anti-Domoic Acid HRP Conjugate in solution. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Domoic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
- Fresh water (0.0065 – 10 ppb)
- Seawater/marine water (0.325 - 500 ppb) – after dilution
- Shellfish tissue (13 – 20,000 ppb or 0.013 ug/100g – 20 ug/100g) - after extraction
Format: 96-Well Plate ELISA
|No. of tests||96 wells|
|Detection limit||Fresh water (0.0065 – 10 ppb) | Seawater/marine water (0.325 - 500 ppb) – after dilution | Shellfish tissue (13 – 20,000 ppb or 0.013 ug/100g – 20 ug/100g) - after extraction|
|Time to Result||2 hours|
|Kit components||1. Microtiter plate coated with Domoic Acid conjugated to a protein, in a resealable foil pouch with desiccant. 2. Domoic Acid Standards (6): 0, 6.5, 22, 254, 865, and 10 000 ppt and Control (1) 75 ppt ± 15 ppt, 1.0 mL each. 3. 10X Sample Diluent, 30 mL, must be diluted prior to use, see Test Preparation (Section C). 4. Anti-Domoic Acid HRP Conjugate Concentrate (2), must be diluted prior to use, see Test Preparation (Section C). 5. Anti-Domoic Acid HRP Conjugate Diluent. 6. Wash Solution (5X) Concentrate, 100 mL, must be diluted prior to use, see Test Preparation (Section C). 7. Color (Substrate) Solution (TMB), 13.5 mL. 8. Stop Solution, 13.5 mL.|