DDE/DDT, ELISA, 96 tests
The DDE/DDT ELISA is an immunoassay for the quantitative and sensitive screening of DDE/DDT. This test is suitable for the quantitative and/or qualitative screening of DDE/DDT in water samples (groundwater, surface water, and well water). For soil and other sample matrices, please contact Abraxis technical services for application bulletins and/or specific matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of DDE/DDT by specific antibodies. DDE/DDT, when present in a sample, and a DDE/DDT-HRP analogue compete for the binding sites of the anti-DDE/DDT antibodies in solution. The DDE/DDT antibodies are then bound by a second antibody (anti-mouse) immobilized on the wells of the microtiter plate. After a washing step and the addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of DDE/DDT present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.