Cylindrospermopsin, ELISA, 96 tests
The Cylindrospermopsin ELISA is an immunoassay for the quantitative and sensitive detection of Cylindrospermopsin in water samples. The test is a direct competitive ELISA for the detection of Cylindrospermopsin. It is based on the recognition of Cylindrospermopsin by specific antibodies. Cylindrospermopsin, when present in a sample, and a Cylindrospermopsin-HRP analog compete for the binding sites of rabbit anti-Cylindrospermopsin antibodies in solution. The anti-Cylindrospermopsin antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Cylindrospermopsin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
|No. of tests||96|
|Detection limit||0.04 ppb (μg/L)|
|Kit components||1. Microtiter plate (12 X 8 strips) coated with a second antibody (goat anti-rabbit) 2. Standards: 0, 0.05, 0.10, 0.25, 0.50, 1.0, 2.0 ppb; 1 mL each 3. Control: 0.75 ± 0.15 ppb, 1 mL, prepared from a secondary source, for use as a Quality Control Standard (QCS) 4. Sample Diluent, 25 mL, for use as a Laboratory Reagent Blank (LRB) and for dilution of samples above the range of the standard curve 5. Cylindrospermopsin-HRP Conjugate Solution, 6 mL 6. Antibody Solution (rabbit anti-Cylindrospermopsin), 6 mL 7. Wash Buffer (5X) Concentrate, 100 mL 8. Substrate (Color) Solution (TMB), 12 mL 9. Stop Solution, 12 mL|