Modified Cry3A LFS kit for Corn

Modified Cry3A LFS kit for Corn

Cry34Ab1 LFS kit

Cry34Ab1 LFS kit

Cry3Bb LFS kit for Corn leaf or seed

Cry3Bb lateral flow strips (LFS) is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Root worm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf.

An antibody specific to the Cry3Bb protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry3Bb molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.

When the LFS is placed in the sample extract, the Cry3Bb protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry3Bb, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.

More Information
SKU AID 054
No. of tests 100
Max no. of samples 100
Specificity Qualitative detection of Cry3Bb protein present in transgenic Maize plant carrying Monsanto Yieldgard Root worm RW event 863. The test is used to detect the Cry3Bb protein in individual Maize seed or leaf.
Relevant species Individual Maize seed or leaf
Cross-reactivities The Cry3Bb LFS kit does not cross react with CP4EPSPS (Roundup Ready**), Cry1F, Cry1Ac, eCry3.1Ab (AgriSure Duracade), mCry3A, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink***).
Storage 2 - 8° C.
Time to Result 5 min
Kit components Cry3Bb LFS strips 50 strips per canister: Two canisters per kit; Extraction buffer:Two vials of 50 ml each (Ready to use); One dropper; One pack insert

Test Instructions