Vip3A LFS kit for Corn bulk grain ( 0.25 %)
Vip3A lateral flow strips (LFS) test kit for bulk grain corn is intended to be used for the qualitative detection of Vip3A protein present in corn. The kit detects one Vip3A corn seed in 400 conventional corn seeds (0.25%)
The total incubation time of the assay is10 minutes.
An antibody specific to the Vip3A protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Vip3A molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.
When the LFS is placed in the sample extract, the Vip3A protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Vip3A, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.
|No. of tests||100|
|Max no. of samples||100|
|Specificity||Qualitative detection of Vip3A protein present in corn. The kit detects one Vip3A corn seed in 400 conventional corn seeds (0.25%).|
|Relevant species||Bulk grain corn|
|Cross-reactivities||The Vip3A test kit does not cross react with CP4EPSPS, Cry1F, Cry1Ac, Cry1Ab, Cry2A, Cry34Ab, Cry35Ab1, Cry3B, mCry3A, eCry3.1Ab and PAT ( Liberty Link***).|
|Assay time||10 min|
|Storage||2 - 8° C|
|Kit components||Vip3A LFS strips 50 strips per canister: Two canisters per kit; One pack insert|