Cry1F LFS kit for Corn/Cotton
Cry1F lateral flow strips (LFS) test kit is intended to be used for the qualitative detection of Cry1F protein present in individual cotton/corn leaf or seed. The total incubation time of the assay is 5 minutes.
An antibody specific to the Cry1F protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry1F molecule is conjugated with colloidal gold and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS. When the LFS is placed in the sample extract, the Cry1F protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry1F, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.
Cross Reactivity: The Cry1F LFS kit does not cross react with CP4EPSPS (RoundUp Ready**), Cry1Ac, eCry3.1Ab (AgriSure
Duracade), Cry3Bb, Cry1Ab, Cry2A, Vip3A, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink***).
|No. of tests||100|
|Max no. of samples||100|
|Specificity||Qualitative detection of Cry1F protein present in individual cotton/corn leaf or seed.|
|Relevant species||Individual cotton/corn leaf or seed|
|Time to Result||5 min|
|Kit components||Cry1F LFS strips 50 strips per canister - Two canisters per kit; Extraction buffer - Two vials of 50 ml each (Ready to use); One pack insert; One Calibrated dropper|