CP4 EPSPS, Dipstick, 50-test
Rapid immunochromatographic test designed solely for use in the qualitative screening to detect the presence of CP4 EPSPS.
Genentically modified seeds that expresses the CP4 EPSPS protein (or Roundup Ready) can tolerate the herbicide glyphosate used by farmers to kill weeds without damaging the crops that have been genetically modified to be resistant to the herbicide. The CP4 EPSPS Strip test is a rapid immunochromatographic test, designed solely for use in the qualitative screening to detect the presence of CP4 EPSPS in corn, cotton, and soybean seeds and leaf samples (please refer to the appropriate sample preparation or extraction). If necessary, positive samples can be confirmed by PCR or other conventional methods.
The lateral flow test is based on a "sandwich" format of the specific immunochemical reaction between a gold-labeled antibody, a protein, and an unlabeled antibody. CP4 EPSPS, if present in a sample solution, will bind with gold-labeled antibodies in the sample pad, forming an antibody-protein complex. The solution wicks up the strip, causing the fluid to pass over an area containing the immobilized antibodies specific to the protein on the nitrocellulose membrane, forming a sandwich (test line). The excess unbound gold-labeled antibodies will continue to migrate up the strip and bind to different immobilized antibodies specific to the gold-labeled antibody, producing a second visible line (control line). If CP4 EPSPS is present in a sample at a quantity above the limit of detection, it will saturate the antibody binding sites, producing a line. If there is no CP4 EPSPS present, it is considered a negative result. Any line developing at the test line is considered a positive result. The control line is not influenced by the presence or absence of CP4 EPSPS in the sample, and therefore should be present in all reactions.
|No. of tests||50|
|Specificity||Qualitative screening to detect the presence of CP4 EPSPS|
|Relevant species||Corn, cotton, and soybean seeds and leaf samples|