Chloramphenicol, ELISA, 96 tests
The Chloramphenicol ELISA is an immunoassay for the detection of Chloramphenicol. This test is suitable for the quantitative or qualitative detection of Chloramphenicol in contaminated samples. Positive samples should be confirmed by HPLC, GC/MS, or other conventional methods. The test is a direct competitive ELISA based on the recognition of Chloramphenicol by specific antibodies. Chloramphenicol, when present in a sample, and a Chloramphenicol-enzyme conjugate compete for the binding sites of monoclonal mouse anti-Chloramphenicol antibodies in solution. The Chloramphenicol antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Chloramphenicol present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.