Carbamazepine, ELISA, 96-test
The Carbamazepine ELISA is an immunoassay for the quantitative and sensitive detection of Carbamazepine in water samples. A pre-sample concentration is not required. If necessary, positive samples can be confirmed by HPLC, or other conventional methods. The test is a direct competitive ELISA that allows the detection of Carbamazepine. It is based on the recognition of Carbamazepine by specific antibodies. Carbamazepine, when present in a sample, and a Carbamazepine-HRP analogue compete for the binding sites of mouse anti-Carbamazepine antibodies in solution. The Carbamazepine antibodies are then bound by a second antibody (goat anti mouse) immobilized in the plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of the Carbamazepine present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.