Bt Cr1Ab/Cry1Ac, Dipstick, 50-test

The Bacillus thurigiensis (Bt) Cry1Ab/1Ac protein is an insecticidal crystalline protein expressed by the Cry1Ab/1Ac gene in certain strains of genetically modified plants. The Bt Cry1Ab/1Ac ELISA is a rapid immunochromatographic test designed solely for use in the qualitative screening of to detect the presence of Bt Cry1Ab/1Ac in corn and cotton seeds, and leaf samples (please refer to the appropriate sample preparation or extraction protocol). If necessary, positive samples can be confirmed by PCR or other conventional methods.

The lateral flow test is based on a "sandwich" format of the specific immunochemical reaction between a gold-labeled antibody, a protein, and an unlabeled antibody. Bt Cry1Ab/1Ac, if present in a sample solution, will bind with gold-labeled antibodies in the sample pad, forming an antibody-protein complex. The solution wicks up the strip, causing the fluid to pass over an area containing the immobilized antibodies specific to the protein on the nitrocellulose membrane, forming a sandwich (test line). The excess unbound gold-labeled antibodies will continue to migrate up the strip and bind to different immobilized antibodies specific to the gold-labeled antibody, producing a second visible line (control line). If Bt Cry1Ab/1Ac is present in a sample at a quantity above the limit of detection, it will saturate the antibody binding sites, producing a line. If there is no Bt Cry1Ab/1Ac present, it is considered a negative result. Any line developing at the test line is considered a positive result. The control line is not influenced by the presence or absence of Bt Cry1Ab/1Ac in the sample, and therefore should be present in all reactions.