BACGene Listeria spp.
The Eurofins BACGene real-time PCR kits are designed for fast and reliable detection of pathogens in food. The BACGene real-time PCR kits offer next day-results and, due to their flexible design, are equally useful for the testing of low and high sample volumes. All kits use an IPC as amplification control.
The kits are certified by AFNOR and by AOAC as Proficiency Tested™ Methods. All kits come with an evaluation software for easy evaluation and documentation of results.The BACGene Listeria spp. kit is designed for fast detection of Listeria spp. with 96 lysis and real-time PCR reactions.
|No. of tests||96|
|Max no. of samples||94|
|Specificity||Listeria spp. (included in NF VALIDATION scope and AOAC validated: L. monocytogenes, L. grayi , L. innocua, L. ivanovii, L. welshimeri and L. seeligeri; additionally AOAC validated for L. marthii and L. rocourtiae)|
|Detection limit||1-3 CFU/25 g|
|Time to Result||21 h|
|PCR cycler platforms||Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well|
For Lysis: - 96 well lysis plate - Domed caps for lysis plate - Lysis buffer L (2x 2,5 mL) - Proteinase K (2x 500 µL) - Lysozyme (2x 500 µL)
For PCR: - 96 well PCR plate with pre-dispensed Mastermix and PCR support grid - Optical caps for PCR plate - Listeria positive control (2x 50 µL)
|AFNOR certification||The BACGene Listeria spp. kit is certified by AFNOR Certification as an alternative method for Listeria spp. detection in all human food products and environmental samples according to EN ISO 16140-2 (EGS 38/02-01/17)|
|AOAC certification||The BACGene Listeria spp. kit is certified by AOAC-Research Institute under the Performance Tested Methods Program for detection of Listeria spp. with certificate no. 061702.|