BACGene Listeria Multiplex 10xHTP
The Eurofins BACGene real-time PCR kits are designed for fast and reliable detection of pathogens in food.
The BACGene real-time PCR kits offer next day-results and, due to their flexible design, are equally useful for the testing of low and high sample volumes. All kits use an IPC as amplification control. The kits are certified by AFNOR and by AOAC as Proficiency Tested™ Methods. All kits come with an evaluation software for easy evaluation and documentation of results.
With the BACGene Listeria Multiplex kit, Eurofins offers the first multiplex kit for simultaneous detection of Listeria monocytogenes and Listeria spp. with 10x96 lysis and real-time PCR reactions. This kit is designed for high-throughput with larger amounts of samples.
|No. of tests||960|
|Max no. of samples||940|
|Specificity||Listeria monocytogenes and Listeria spp.
NF VALIDATION scope and AOAC validated for: L. monocytogenes, L. grayi, L. innocua, L. ivanovii, L. welshimeri and L. seeligeri; additionally AOAC validated for L. marthii and L. rocourtia.
|Detection limit||1-3 CFU/25 g|
|Time to Result||21 h|
|PCR cycler platforms||Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well|
For Lysis: - 10x 96 well lysis plate - 10x Domed caps for lysis plate - Lysis buffer L (2x 25 mL) - Proteinase K (2x 5 mL) - Lysozyme (2x 5 mL)
For PCR: - 10x 96 well PCR plate with pre-dispensed Mastermix and PCR support grid - 10x Optical caps for PCR plate - Listeria positive control (4x 50 µL)
|AFNOR certification||The BACGene Listeria Multiplex kit is certified by AFNOR Certification as an alternative method for Listeria monocytogenes and Listeria spp. detection in all human food products and environmental samples according to EN ISO 16140-2. The end of the validity of the NF VALIDATION certification is indicated on the certificate EGS 38/05-03/17.|
|AOAC certification||The BACGene Listeria Multiplex kit is certified by AOAC-Research Institute under the Performance Tested Methods Program for detection of Listeria monocytogenes and Listeria spp. with the certificate no. 061701.|