Anatoxin-a (VFDF), ELISA, 96 tests
The Anatoxin-a ELISA is an immunoassay for the quantitative and sensitive detection of Anatoxin-a in water samples. The test is a direct competitive ELISA based on the recognition of Anatoxin-a by a monoclonal antibody. Anatoxin-a, when present in a sample, and an Anatoxin-a-enzyme conjugate compete for the binding sites of mouse anti-Anatoxin-a antibodies in solution. The Anatoxin-a antibodies are then bound by a second antibody (anti-mouse) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anatoxin-a present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
|No. of tests||96|
|Detection limit||0.1 ppb (μg/L)|
|Kit components||1. Microtiter plate coated with a secondary antibody (anti-mouse), in a re-sealable aluminum pouch 2. Lyophilized Anatoxin-a-HRP Enzyme Conjugate, 3 vials 3. Conjugate Diluent, 12 mL 4. Lyophilized Anti-Anatoxin-a Antibody, 3 vials 5. Antibody Diluent, 12 mL 6. Empty clear and amber HDPE bottles 7. (+)Anatoxin-a Standards: 0, 0.15, 0.40, 1.0, 2.5, 5.0 ppb; 1 mL each 8. Control at 0.75 +/- 0.185 ppb, 1 mL 9. Sample Diluent (10X) Concentrate, 2 X 25 mL 10. Wash Buffer (5X) Concentrate, 100 mL 11. Substrate (Color) Solution (TMB), 12 mL 12. Stop Solution, 12 mL|